Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_526cc127737ea2caabcd81e2dafe3972 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-52 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-245 |
filingDate |
2017-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3db17cc0f3346270a5a14b77ae2976eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_367046fa8467d68dd998d1f8e588683a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb9781f2d1003d2c14a8cef1e8c04dbb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a2f6e597d178b6d71bea3fcc5c8ce80 |
publicationDate |
2017-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2017283469-A1 |
titleOfInvention |
Proteolytic inactivation of select proteins in bacterial extracts for improved expression |
abstract |
The present disclosure provides modified proteins that are capable of being cleaved by the protease OmpT1. The proteins can be modified in an exposed surface motif to incorporate OmpT1 cleavage sites. Also provided are nucleic acids encoding the modified proteins, bacterial cells that express the modified proteins, and cell free synthesis systems containing modified RF1. The disclosure further provides methods for reducing the deleterious activity of a modified protein in a cell free synthesis system by contacting the modified protein with OmpT1. Also provided are methods for reducing RF1 competition at an amber codon in the cell free synthesis system, and methods for expressing a protein in the cell free synthesis system. The modified proteins of the invention can be used to increase the yield of proteins having non-natural amino acids incorporated at an amber codon. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021050554-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022076853-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021091986-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022076859-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021030706-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11077195-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11622993-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021263026-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11701407-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023122750-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023122573-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10610571-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022256538-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022256534-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022174102-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022174101-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11261219-B2 |
priorityDate |
2012-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |