http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2017184581-A1
Outgoing Links
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|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e3d6fa926ef6ac3947565c2d90afae18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8b4ee13af463c18c765406625e1c5d44 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-05 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2291-0422 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54373 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N29-032 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-553 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6825 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N29-032 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-553 |
| filingDate | 2017-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e54acecb614b5dbd787c6cc0ac991f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fcf4326b6c2c0eaefec22a9c7d836286 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_868acd46e02e78d46921b28a20ac2d56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5cb9d1801204592fc49e42dec466ae6d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d00538504fb0d0a28a0bac620cc6cd7c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da8c5e5de1e22755dd09a040e6ecacdd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d2aa81cc38077c583a884eb609c662e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8694e89cd419bb4950b653da765c6ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4960c724556a637d2b4efd5b3455d7fb |
| publicationDate | 2017-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2017184581-A1 |
| titleOfInvention | Detection of bioagents using a shear horizontal surface acoustic wave biosensor |
| abstract | Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×10 4 -fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11203702-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10031140-B1 |
| priorityDate | 2007-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 191.