http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2017175156-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c4626a2a79d0d2572e83fcf56caa84a3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fb5ac5a3db2a0f963f25ed2061bba642 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_00d6ceac4d38cf7d8cabd5004cfd6545 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2304e8417060cea10c40466801fed62c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_24b9717b56b3fa1426ce6f3d27f7d29f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2016-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58f2c90709d89be200e91c2c6f278d4a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3f3011fef7f40ed8de27df28cf71e42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2995245b650d79cc0df34c909585ed4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea9c5f8220e91264c528f1b91dd7eb87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cafa08cbd083b1701a3cf6a6f885acfd |
| publicationDate | 2017-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2017175156-A1 |
| titleOfInvention | In vitro method for fast scarless DNA assembly using thermostable exonucleases and ligase |
| abstract | The present invention provides a method for scarless in vitro DNA assembly using thermostable exonucleases and ligase, which relates to the field of genetic engineering. The present invention provides a fast method for assembling DNA subfragments with homologous ends, which employs thermostable polymerases and ligase in a thermal cycle of denaturation, annealing, digestion and ligation. After denaturation, DNA subfragments are assembled together via annealing of the homologous end sequences, the unpaired single-stranded overhangs are digested by polymerases, and the resulting nicked gaps are sealed by a ligase. Using this method, 2-6 DNA subfragments were successfully assembled within two hours. This method can be used in conventional DNA recombination and be adapted to high throughput assembly operations. In addition, combinatorial mutations can be easily introduced into the assembled sequence by use of primers with mutated bases. It is particularly suitable for making enzyme and synthetic pathways mutation libraries with high diversity, which can be used in directed evolution to screen for enzymes and synthetic pathways with desirable properties. |
| priorityDate | 2015-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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