| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c8c293fd340e0c8b6a445f7633453a0 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-154
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0864
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-087 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1017
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L7-52 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate |
2016-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58a7ddb684966eac1afdb89de47881e6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c5683baf7c454587d1d1ed8359dc0c6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eca5ce1a15aaba1da0e5b022e326445b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8409b0ed9673fb7e820410df12b70426
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7dd3732b0f936984e2620aa2959ef436
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62175326f5da225bf7479ce4773f56b7 |
| publicationDate |
2017-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2017137871-A1 |
| titleOfInvention |
Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge |
| abstract |
In various embodiments methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulfonating the bound deaminated nucleic acid and/or simultaneously eluting and desulfonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11725251-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113930499-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11260387-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2021108255-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11410750-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11207674-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023077722-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11685958-B2 |
| priorityDate |
2015-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |