http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2017107565-A1

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publicationDate 2017-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2017107565-A1
titleOfInvention Functionalized particles for label-free dna impedimetric biosensor for dna and rna sensing
abstract In one embodiment, 3-Aminopropyltriethoxysilane (APTES) functionalized graphene oxide (APTES-GO) wrapped SiO 2 particle composite (SiO 2 @APTES-GO) was prepared via the self-assembly process of APTES-GO sheets and SiO 2 particles. Transmission electron microscopy (TEM) and Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR) confirmed wrapping of the SiO 2 particles by the APTES-GO sheets. A biosensor based on electrochemical impedance spectroscopy (EIS) was constructed and used to sensitively detect dengue DNA and dengue RNA via primer hybridization using different oligonucleotide sequences. The results demonstrated that the SiO 2 @APTES-GO electrode material led to enhanced sensitivity, selectivity and detection limit, compared to both APTES-GO and APTES-SiO 2 . The three-dimensional structure, high surface area, electrical properties and the ability for rapid hybridization offered by the SiO 2 @APTES-GO rendered this electrode material as ideal to use in the reported dengue impedimetric sensor.
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