| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_12f65d912a87eb3a6e57ebdf37e61dd3 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6839
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2016-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_773fcd63851d60c427977c1f47025cdd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_119d9a94fb29241f16cffd4dab85dea4 |
| publicationDate |
2017-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2017073748-A1 |
| titleOfInvention |
Methods and compositions for genomic target enrichment and selective dna sequencing |
| abstract |
It has been established that one or more large double stranded DNA fragments (each 2,000 to 40,000 base pairs in size) can be captured and isolated from genomic DNA fragments using sequence specific PNA hybridization probes. Compositions and methods for enrichment of a multiplicity of long DNA sequences selected from the genome of any eukaryote are provided. Capture is performed using multiple PNA molecules with gamma-modified chiral backbones, comprising a mixture of neutral and positive chemical groups. Two or more PNA probes with covalently bound haptens, preferably biotin, target each DNA domain of interest for capture, isolation, and subsequent sequencing analysis of the multiplicity of enriched targets, including DNA methylation sequencing. The methods include enhancement of probe-DNA binding specificity through single strand binding proteins (SSB). |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107794575-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11242526-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110981929-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102356360-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20190046787-A |
| priorityDate |
2015-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |