| abstract |
The present invention relates to a pincer for binding to a target material, wherein an antibody or a fragment thereof binding to a first target site of the target material and an aptamer binding to a second target site are conjugated via a linker, a preparation method thereof, a composition for detecting or separating the target material comprising the pincer, a kit for detecting or separating the target material comprising the composition, and a method for detecting or separating the target material using the kit. n Also, the present invention relates to a drug carrier comprising the pincer for binding to a target material, wherein an aptamer conjugated to a second target site of the pincer loads the drug. n The antibody-aptamer pincer comprising the antibody and the aptamer, which target for the different binding sites on the same material of the present invention shows a ten- to hundred-fold increased binding affinity for the target molecule compared to using the antibody or the aptamer alone, and thus can be usefully applied for detection and/or separation of traces of the target material. n Further, the affinity of the drug carrier prepared by the binding of the antibody with the aptamer for the target molecule is increased, and thus, it can be used as a drug carrier against harmful tumors. |