| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_af18812449a9f384c3c9f5c128d7f09e |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2014-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2ffac06d660d5b47c092db20dd66f32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_308f7f18abd38137319529cee0f2b418
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4bf5a923da5756f2be67a64b3ba40004
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af5e7f9528e557a57ec50bdded73c9b4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d4b2c1ad478537d3f10bcc71588c7eff |
| publicationDate |
2017-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2017009302-A1 |
| titleOfInvention |
Method and kit for non-invasively detecting egfr gene mutations |
| abstract |
The present invention discloses a method for non-invasively detecting EGFR gene mutations in subjects, comprising the following steps: designing primers according to EGFR gene exons; extracting plasma DNAs in subjects; connecting the extracted plasma DNAs with tagging linkers; PCR pre-amplifying the tagging linkers connected plasma DNAs; cyclising the pre-amplified DNAs to obtain cyclised DNAs; PCR amplifying the cyclised DNAs using the designed primers; and high throughput sequencing the PCR amplified product and analyzing the EGFR gene mutations. The present invention also discloses a corresponding kit. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111518901-A |
| priorityDate |
2013-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |