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publicationDate 2016-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2016356716-A1
titleOfInvention Method for detection of a reversibly photo-convertible fluorescent species
abstract Method for detection of at least one reversibly photo-convertible fluorescent species (P), comprising the following steps: a) illumination of a sample comprising said or at least one of said reversibly photo-convertible fluorescent species by a periodically modulated illuminating light (FEX); and b) detection of fluorescent emission (FLU) emitted by the sample thus illuminated; characterized in that the method further comprises the following step: c) extraction of the amplitude (IFOUt) of the intensity component of said fluorescent emission exhibiting the same periodicity as said periodically modulated illuminating light and a phase quadrature with respect to the same; and in that the mean intensity of said illuminating light and the modulation frequency of the same are chosen in such a way as to maximise said amplitude of the intensity component of said fluorescent emission.
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