Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_680dd0699e3f4ba8532d128c7152b3bb |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-112 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-143 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6811 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6832 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate |
2016-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3839450be3518a31552bfa9721402ca0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5bd97cd4df01458953fade1f693c831 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae1addc8faa1c1ad2018d938d8cf8d90 |
publicationDate |
2016-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2016340727-A1 |
titleOfInvention |
Fine-tuned ultraspecific nucleic acid hybridization probes |
abstract |
Compositions and methods for highly specific nucleic acid probes and primers are provided. The probe system comprises a complement strand and a protector stand that form a partially double-stranded probe. The reaction standard free energy of hybridization between the probe and target nucleic acid as determined by Expression 1 (ΔG° rxn =ΔG° t-TC −ΔG° nh-PC +(ΔG° v-TC −ΔG° h-PC )) is from about −4 kcal/mol to about +4 kcal/mol. Alternatively, the reaction standard free energy of hybridization between the probe and target nucleic acid is determined by Expression 1 to be within 5 kcal/mol of the standard free energy as determined by Expression 2 (−Rτ ln(([P] 0 −[C] 0 )/[C] 0 )]), where the [P] 0 term of Expression 2 equals the concentration of the protector strand and the [C] 0 term of Expression 2 equals the concentration of the complement strand. In addition, a method for on-the-fly fine tuning of a reaction using the present probe is provided. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11505819-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11414686-B2 |
priorityDate |
2013-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |