| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_00d8a292f9cb8de0ddb88f6e78420e53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1c8cbbf2290b3f3a9988da29598baa2c |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-043
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502753
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L7-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-0668
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-10 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L7-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L7-00 |
| filingDate |
2016-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f81c0b6d81f3a0ccd62ec33810357451
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dcb6e16c6a3af1d246eb723c5bf5d1d4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1fdd030c3345d8f5eff58217e1391db6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2735ca3729aa2c0b941749ed912c6ea5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3c9fe06fd39d79ba4a695ae666f772c |
| publicationDate |
2016-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2016340668-A1 |
| titleOfInvention |
Single-step dna preparation for polymerase chain reaction using magnetic chitosan microparticles |
| abstract |
The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113293159-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112881680-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108251512-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114029041-A |
| priorityDate |
2015-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |