http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2016304942-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8073450a491d200d05b09b9beb6f0ec6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f210a9e983d8d7437cc59e03bac79ee6 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6832 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2014-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aab1e71acd58c7d288017d1b4d4cd662 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e288afa6165bbd7e5a313c9feed15ed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b46de02afded89f504ad0b4e22ac5d49 |
| publicationDate | 2016-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2016304942-A1 |
| titleOfInvention | Enhanced Methods of Ribonucleic Acid Hybridization |
| abstract | This disclosure relates to a method for increasing the hybridization efficiency of a probe and a target RNA in a sample, for example to identify a particular RNA present in the sample. The method includes heating a lysate sample comprising at least one target RNA, such as a tRNA, mRNA or rRNA, at a temperature of about 95° C. for a time sufficient to interfere with secondary structure of the RNA, wherein the time is short enough, such that the RNA in the cell lysate sample are not significantly degraded, and wherein the lysate comprises a cell lysis buffer comprising a chemical denaturant. To detect a target RNA in the lysate, the lysate is contacted with at least one detectable probe, such as a labeled probe, designed to specifically hybridize to the target RNA in the lysate. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020124050-A1 |
| priorityDate | 2013-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 963.