| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc2b03342511abc88f60c63f2acd96d0 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54373
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-553 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-553
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 |
| filingDate |
2014-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23687bb7825d78fbec44205929a15cb6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6e7843612cde653340bf26d16d0d5c3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d14a1f04bf51b4a61f71ca6c5d5df9a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c9581989432e0f515f73280add7a4b9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c6dc12bce0cf7b4f7f8481f20e57147 |
| publicationDate |
2016-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2016216252-A1 |
| titleOfInvention |
Plasmonic beads for multiplexed analysis by flow detection systems |
| abstract |
Disclosed are methods and assays for detection of low concentration analytes such as proteins in a sample, using beads. Specially coated beads allow for femtomolar sensitivity through strong near-infrared fluorescence enhancement on plasmonic beads having gold nanostructures in a coating. By selecting different bead sizes and labeling with different fluorophores of plasmonic beads for immobilization of different capture antibodies, multiplexed plasmonic beads can be used for simultaneous quantification of various markers down to 0.01 pg/mL sensitivity. Exemplified are human cytokine IL-6, IFN-gamma, IL-1 beta, VEGF and ovarian cancer biomarker CA-125. Using flow cytometry, a detection limit below that of glass bead based immunoassays by 2-3 orders of magnitude was achieved. The multiplexed plasmonic bead assay was used to simultaneously quantify cytokines and CA125 of ovarian cancer cell culture medium, demonstrating the potential of plasmonic bead based immunoassay for sensitive biological detection relevant to human diseases. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10876955-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2018100802-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019066323-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11193890-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10641710-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11536719-B2 |
| priorityDate |
2013-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |