Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69f43505d1b3ec31c224b32b5d1ec4e2 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K2-00 |
filingDate |
2014-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be493a977d8dff7534bda2610d396e68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dea49d46495b6ca328cd39f913760e47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a55e53524618ab81e41fcc284a768530 |
publicationDate |
2016-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2016215034-A1 |
titleOfInvention |
Water-soluble expression and purification method of human gcsf recombinant protein having biological activities |
abstract |
The present invention relates to a water-soluble expression and a purification method of a human granulocyte colony-stimulating factor (hGCSF) recombinant protein having biological activities using an N-terminal tag, and provides a recombinant expression vector containing a solubility enhancing tag and a hGCSF gene. In addition, provided are: a recombinant microorganism transformed with the recombinant expression vector; a method for producing a hGCSF recombinant protein (rhGCSF); and a rhGCSF produced by the production method. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109517074-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111378047-A |
priorityDate |
2013-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |