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filingDate 2016-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2016-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2016206971-A1
titleOfInvention Temperature-assisted on-column focusing
abstract A method of comprising:n introducing a sample volume into an inlet end of a liquid chromatography column, wherein the liquid chromatography column includes a focusing segment proximal to the inlet end of the liquid chromatography column and a separation segment proximal to an elute outlet of the liquid chromatography column; maintaining only the focusing segment at a first temperature as the sample is introduced into the focusing segment; and subsequently heating the focusing segment to a second temperature that is higher than the first temperature after the entire sample volume has been introduced into the focusing segment.
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