http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2016152656-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fc06cad2b58bbfcc30e3c96a6eb6c926 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 |
| filingDate | 2014-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f992fabca1f2911602298fbedff166ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40fd2f950e8ea3e4dbb9db9ec1b5c635 |
| publicationDate | 2016-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2016152656-A1 |
| titleOfInvention | Methods of Using Phosphoramidation Reaction for Conjugation-Labeling Nucleic Acids |
| abstract | Methods are provided to label nucleic acids through two-step phosphoramidation reactions with regiospecificity and high sensitivity. The methods can be widely used for synthesizing conjugates of nucleic acids. Through the two-step phosphoramidation reactions, nucleic acids are directly or indirectly labeled for easier and more efficient applications. The present invention also uses fluorescence quenching and DNA melting profile studies to demonstrate that the nucleic acids modified through the two-step phosphoramidation reactions preserve intrinsic base-pairing specificity and bestow regioselectivity in nucleic acids with high yield. Thus, the present invention has significant contribution to basic nucleic acid research; and, in the future, can be applied to effectively and quantitatively detect and measure nucleic acids inside and outside living organisms. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111808988-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114315938-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110702899-A |
| priorityDate | 2014-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.