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publicationDate 2016-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2016130642-A1
titleOfInvention Method, microreactor and apparatus for carrying out real-time nucleic acid amplification
abstract A method for carrying out nucleic acid amplification, includes providing a reaction chamber, accommodating an array of nucleic acid probes at respective locations, for hybridizing to respective target nucleic acids; and introducing a solution into the reaction chamber, wherein the solution contains primers, capable of binding to target nucleic acids, nucleotides, nucleic acid extending enzymes and a sample including nucleic acids. The a structure of the nucleic acid probes and of the primers so that a hybridization temperature of the probes is higher than an annealing temperature of the primers, whereby hybridization and annealing take place in respective separate temperature ranges.
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