http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2016122720-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_716e9a2606ffd4ba1a2a3917e4a402fd |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-603 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-604 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-602 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-1307 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-60 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0696 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074 |
filingDate | 2014-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44abf54d3aa86537a3363ec8e294c68f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06fbb5a477a5967ff1ea8020ad5c1e4c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4997547098912ce385aeb04f1f56699 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a71c0781baf1971f7f5f8af791143e6 |
publicationDate | 2016-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2016122720-A1 |
titleOfInvention | Method of efficiently establishing induced pluripotent stem cells |
abstract | The invention provides a method of producing iPS cells, which comprises the steps of (i) introducing reprogramming factors into somatic cells; (ii) culturing the cells obtained in step (i) for more than 11 days and not more than 29 days; (iii) sorting TRA-1-60-positive cells from the cells obtained in step (ii); (iv) culturing the TRA-1-60-positive cells sorted in step (iii); (v) transferring a colony obtained in step (iv) to another culture vessel; and (vi) culturing the cells obtained in step (v), thereby obtaining iPS cells. The cells obtained in step (v) are preferably subcultured 10 times or more. The invention also provides a method of producing a population of differentiated cells that has a reduced rate of residual undifferentiated cells, which comprises inducing differentiation of the iPS cells obtained by the above-mentioned method. |
priorityDate | 2013-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 635.