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filingDate 2015-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2016-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2016083736-A1
titleOfInvention Method of assembling multi-fragment dna molecule and use thereof
abstract A method of assembling multi-fragment DNA molecule, the method including: 1) constructing a vector system including an acceptor vector A1, a first donor vector B1, and a second donor vector B2, and each of the acceptor vector A1, the second donor vector B2, and the second donor vector B2 including a multiple cloning site; 2) introducing a first target sequence L m to the first donor vector B1, m being an odd number starting from 1, and introducing a second target sequence L n to the second donor vector B2, n being an even number starting from 2; and 3) repeating 2) to assemble multiple fragments to yield a multi-fragment DNA molecule.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106222165-A
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