http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2016076023-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_37b416a8c0d2b0f1c3d6026d932027af |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1065 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2014-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ce8c3994db2241758dde4efbfc521a6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1254367c91e0271e9930be473a95d5b2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5a0aca4822ce14c9c4aa30433c36ed4 |
publicationDate | 2016-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2016076023-A1 |
titleOfInvention | Method for generating extended sequence reads |
abstract | The present invention provides an approach to increase the effective read length of commercially available sequencing platforms to several kilobases and be broadly applied to obtain long sequence reads from mixed template populations. A method for generating extended sequence reads of long DNA molecules in a sample, comprising the steps of: assigning a specific barcode sequence to each template DNA molecule in a sample to obtain barcode-tagged molecules; amplifying the barcode-tagged molecules; fragmenting the amplified barcode-tagged molecules to obtain barcode-containing fragments; juxtaposing the barcode-containing fragments to random short segments of the original DNA template molecule during the process of generating a sequencing library to obtain demultiplexed reads; and assembling the demultiplexed reads to obtain extended sequence reads for each DNA template molecule, is disclosed. Also disclosed are methods systems and software for assembling paired end sequence reads to produce extended reads. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10233490-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112534507-A |
priorityDate | 2013-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 102.