Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c7c3061f81f6e039ff26d8b897617c05 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2511-00 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0654 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0632 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0664 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-32 |
filingDate |
2015-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e8eaf99ac5981a221bef542ecd0502a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a10609f2ae0a4b4fe2aca545bdd82f93 |
publicationDate |
2016-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2016053228-A1 |
titleOfInvention |
Isolation, expansion and characterization of precursor/stem cells from dental tissues |
abstract |
A method for isolating and proliferating at least one type of precursor cell from dental origin from a single donor includes the following isolating the precursor cells from dental origin of a single donor in a sample collection media and preparing a primary stock culture. The primary stock culture is proliferated sequentially to obtain first, second and third sub-cultured stocks with cell counts ranging between 5×10 6 cells and 10×10 6 cells, 20×10 6 cells and 400×10 6 cells, 150×10 6 and 300×10 6 cells respectively. The precursor cells from the third subculture are harvested and cryo-preserved to obtain a precursor cell population for cell transplantation. The dental origin of the precursor cells is from pulp, apical papilla or periodontal ligament. The precursor cell originates from mesenchymal stem cells, ecto-mesenchymal cells, neural stem cells, dental progenitor cells or CD117 + cells. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109819975-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109628389-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111004774-A |
priorityDate |
2014-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |