http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2016041185-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc2b03342511abc88f60c63f2acd96d0 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-4716 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-564 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2014-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40545ed669ee33162256a98b0c53fc5e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_25ba5e47e1743678d92c17114d5988a4 |
| publicationDate | 2016-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2016041185-A1 |
| titleOfInvention | Methods of Detecting Complement Fixing and Non-Complement Fixing Antibodies and Systems for Practicing the Same |
| abstract | Provided are methods for simultaneously determining the presence or absence of complement-fixing antibody (CFAb) and non-complement-fixing antibody (non-CFAb) in a biological sample in a single reaction. The methods include mixing a cellular sample from a donor, a biological sample from a recipient, isolated human complement component C1q (C1q), and a labeled C1q binding agent (CBA), under conditions sufficient for recipient CFAb and non-CFAb, if present, to bind to donor cell surface antigen (Ag) to form antibody-Ag (Ab-Ag-C1q-CBA) complexes. The methods further include contacting the mixture with labeled an-ti-hIgG antibodies and labeled anti-CD antibodies, and detecting (e.g., semi-quantitatively) the presence or absence of the detectable labels bound to the antibody-Ag complexes to determine the presence or absence of CFAb and non-CFAb in a biological sample from the recipient. Systems and kits for practicing the subject methods are also provided. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10527613-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10746744-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10338080-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10101337-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11079373-B2 |
| priorityDate | 2013-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 167.