Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1b5d47c4f1d77d82de7ce1f64ba985b3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1b0c198757da18cf77dfbdcef00abb70 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502784 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502784 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N35-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2014-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f90a6a768fb254ae96142258754c0c6b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f385985cd3de6a963746b787329c23aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94ab34fbfe45ae5fa465811d889014f1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad20ffe428bc91f23826cece3c3dc2da http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5c1a894e1b81e8f05a82aafde2e1388 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1521a23a2a953502db2037475171e2a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fbf2ee617dca07177cb1978c9aa5ed5a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b2b5e7071a3f009c07f1f732f30324d |
publicationDate |
2016-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2016040224-A1 |
titleOfInvention |
Single nucleotide detection method |
abstract |
A method for determining the sequence of nucleotide bases in a polynucleotide analyte is provided. It is characterised by the steps of (1) generating a stream of single nucleotide bases from the analyte by pyrophosphorolysis; (2) producing captured molecules by reacting each single nucleotide base with a capture system labelled with detectable elements in an undetectable state; (3) releasing the detectable elements from each captured molecule in a detectable state and (4) detecting the detectable elements so released and determining the sequence of nucleotide bases therefrom. The method can be used advantageously in sequencers involving the use of microdroplets. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3912725-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10525459-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10501795-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2018057872-A1 |
priorityDate |
2013-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |