http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015361143-A1

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inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f286f4cc5d767e7fb6389e9da1d512d5
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publicationDate 2015-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2015361143-A1
titleOfInvention Method for producing high-quality recombinant allergens in a plant
abstract A method for producing a recombinant protein in a plant, in particular a tobacco plant, preferably Nicotiana benthamiana , includes the following steps: a) culturing the plant aeroponically or hydroponically, preferably on mobile floats and under LED lighting; b) vacuum agroinfiltration of the plant obtained in a) by agrobacteria that include a DNA fragment coding for the recombinant protein; c) returning the plants to culturing after step b), under the same conditions as for step a); and d) extracting and purifying the recombinant protein from the aerial portions of the plants produced in step c).
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