| abstract |
There is provided a device ( 1 ) for carrying out an assay to detect a target nucleic acid in a sample, the device ( 1 ) comprising a body in which is formed: • a) a sample entry well location comprising a sample entry well ( 22 ) or means for engaging with a sample entry well ( 22 ), the sample entry well ( 22 ) having a first volume; • b) an amplification well location comprising an amplification well ( 56 ) or means for engaging with an amplification well ( 56 ), the well ( 56 ) having a second volume less than or the same as the first volume and in which a nucleic acid amplification reaction of the target nucleic acid may be effected; • c) a first channel ( 60 ) linking the sample entry well ( 22 ) with the amplification well ( 56 ); • d) a diluent well location comprising a diluent well ( 35 ) or means for engaging with a diluent well ( 35 ), the well ( 35 ) being sealed, having a third volume greater than the second volume and being openable to an unsealed configuration; • e) a second channel ( 65 ) linking the diluent well ( 35 ), when in an unsealed configuration, with the amplification well ( 56 ); • f) a third channel ( 70 ) extending from the amplification well ( 56 ); and • g) a lateral flow device ( 80 ) arranged to receive sample from the third channel ( 70 ) and detect the target nucleic acid therein. |