Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d35a75ea1639ce34088fa3e7c520917f |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-10222 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-10022 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-10 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-447 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6872 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-48721 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-487 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2011-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a83d4f78205a810584238e4109e696b1 |
publicationDate |
2015-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2015267253-A1 |
titleOfInvention |
Membrane-integrated viral dna-packaging motor protein connector biosensor for dna sequencing and other uses |
abstract |
Compositions and methods are disclosed that exploit the unprecedented modification of double-stranded DNA virus DNA-packaging motor protein connector polypeptides to render them capable of stable incorporation into lipid membranes as a self-assembled homodocamer that forms an aperture through which conductance can occur when an electrical potential is applied across the membrane. The aperture permits use of the modified protein as a biosensor, for dsDNA sequencing, SNP detection and highly sensitive affinity capture and fingerprinting of analytes, and also finds use in electropotential-driven solute translocation, such as for liposomal loading to form therapeutic nanoparticles (e.g., gene delivery) and bioreactors, and for other uses. The aperture can further be used in optical detection of dsDNA or other acceptor labeled analytes in a fluorophore donor labeled single pore channel. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109069662-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3752284-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019157365-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112119033-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3844506-A4 |
priorityDate |
2008-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |