| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_195baf08e69fc1ec6bbd5ad434cb1c4d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b2c043738199f30b3bcb3d95b3f2820f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c41171ebf75ab6a8fb5c98393b09b8b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f789153dacadbc910962362032722d86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_15b43c136d0e4e6533796cd6420b1f48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dabdf917e2432e32e0338355c06da166
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_68470742c37db68ce35b8ae425ce50cc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2c3083b6d4217828c332048464192333
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a8493dadb4feae4a8de1df43095c3d9e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e822325c7517556550e7bf8656aafaf4 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-924
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-916
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-726
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
| filingDate |
2012-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f12918b1fb93146a85fe5631749d6d9a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_12e11cf8504432081e6e0b6378e05c28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_135c1cc6d8af7b6619e8400cbe95002f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4344c99fac0f035663347de188c2341b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cdc069d7e2f0e4fede5f3c6384e38e6a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_126acc3cd0f4d6b8f459c895de69f91c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23356c18d94a7f925417b32247ef8c2a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5858fa6870416241c717a111eed9b27b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58bc1f13611ba1a37cfff00c2a94f700 |
| publicationDate |
2015-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2015260724-A1 |
| titleOfInvention |
Cell based sensor |
| abstract |
Cell based sensor. The present invention relates to a novel cell based sensor useful for drug discovery that comprises a cell line with professional regulated exocytosis of secretory granules transfected with a non-protease hydrolase as a reporter polypeptide stored in the regulated secretory granules of the cell line with professional regulated exocytosis and having either an endogenous or a heterologous molecule as a modulator of regulated secretory granules exocytosis. Said granule stored non protease-hydrolase reporter having at least: a high resistance to conditions already present inside the granules such as low pH and proteolysis by other proteases; enzymatic activity after exocytosis; a highly specific substrate; absence of toxicity when cell thawing; a very low level of secretion under unstimulated or basal conditions; and a high signal to background activity in a media compatible with cell culture viability and granule exocytosis for a high throughput robust and sensitive detection. |
| priorityDate |
2012-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |