| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_24f54aefd7a75822ce9d6a7759e20068 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01004 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16C20-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1034
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B35-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01004
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B30-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1089
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2437
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B35-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B35-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-02 |
| filingDate |
2015-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_055884b807f33c6d9b5417e13116489d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b509d66d014f7c93e39a58b37fe76f0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_09c7c897c9ba86818cacb595a38cf70f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2a6b1816b3f13f7d2ebe30e7876f4c2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b13e8f61c4441e618b07b4e656b8b9b |
| publicationDate |
2015-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2015259659-A1 |
| titleOfInvention |
Method for obtaining natural variant of enzyme and super thermostable cellobiohydrolase |
| abstract |
A method for selectively obtaining a natural variant of an enzyme having activity includes (1) a step of detecting an ORF sequence of a protein having enzyme activity from a genome database including base sequences of metagenomic DNA of environmental microbiota; (2) a step of obtaining at least one PCR clone including the ORF sequence having a full length, a partial sequence of the ORF sequence, or a base sequence encoding an amino acid sequence which is formed by deletion, substitution, or addition of at least one amino acid residue in an amino acid sequence encoded by the ORF sequence, by performing PCR cloning on at least one metagenomic DNA of the environmental microbiota by using a primer designed based on the ORF sequence; (3) a step of determining a base sequence and an amino acid sequence which is encoded by the base sequence for each PCR clone obtained in the step (2); and (4) a step of selecting a natural variant of an enzyme having activity by measuring enzyme activity of proteins encoded by each PCR clone obtained in the step (2). |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3225688-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10435680-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2017275606-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113736807-A |
| priorityDate |
2014-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |