Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_63f48ec883d93a86a2e5b588cceaaa34 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
filingDate |
2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_09f124c2c6e6f3377ca82228ede688a4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0ef656b79d5da0e6f502433d4a52b70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a812bec467773d97bdf514f6e317ee43 |
publicationDate |
2015-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2015225730-A1 |
titleOfInvention |
Methods for generating libraries with co-varying regions of polynuleotides for genome modification |
abstract |
The invention provides methods for introducing co-varying paired nucleic acids into a vector such under separate transcriptional control. An oligonucleotide is synthesized including the paired nucleic acids. The oligonucleotide is then assembled with a spacer nucleic acid encoding a promoter. After assembly the spacer nucleic acid is to one side of the oligonucleotide encoding the paired segments. However, on circularization of the assembled nucleic acid and cleavage between the DNA segments the spacer oligonucleotide and its components now occur between the paired segments. The resulting nucleic acid can now be cloned into a vector with a single step, such that each of the paired nucleic acid segments is linked to its own promoter. The present method can readily be extended to library screening without proportionately increasing the effort. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107937429-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2020017883-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110249049-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11400110-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3821020-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11643653-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106967716-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11642363-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107090466-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11471530-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11471531-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11466271-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11612617-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106637421-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11130955-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017049129-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019023291-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11098305-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11485973-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11351252-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11547716-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11421227-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11517582-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112703250-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11788085-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11261439-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018053457-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017069829-A3 |
priorityDate |
2014-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |