| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1470af2d007afa44390c8c9cfbaff5f1 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate |
2014-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73037654c5b0fa4a6490e5d0a0b08ec7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca756fa5c048565e8d720494c6a6d019
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10599f16046709ba04e20897a02a5156
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2069c19698399667cd2348b1213ca73a |
| publicationDate |
2015-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2015184215-A1 |
| titleOfInvention |
Development of the soluble recombinant crm197 production by e. coli |
| abstract |
A method for recombinant production of a CRM197 protein includes culturing a recombinant Escherichia coli cell to produce said CRM197 protein, and isolating said CRM197 protein. The recombinant Escherichia coli cell includes an expression vector, which contains a nucleic acid molecule that encodes a fusion protein that includes an E. coli periplasmic signal peptide at N terminal and the CRM197 at C terminal. The CRM197 is encoded by a polynucleotide having the sequence of SEQ ID NO: 1. The E. coli periplasmic signal peptide comprise a pelB leader sequence. The nucleic acid molecule comprises the sequence of SEQ ID NO:2. The Escherichia coli cell is BL21(DE3)pLysS. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112513066-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106350527-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10093704-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11060123-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114075523-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10597664-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20190113440-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3724212-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102048456-B1 |
| priorityDate |
2013-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |