http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015159185-A1

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filingDate 2013-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2015-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2015159185-A1
titleOfInvention Intermittent application of reduced nitrogen sources for selection of PHB producing methanotrophs
abstract A method of selection of polyhydroxybutyrate (PHB) producing Type II methanotrophs is provided that includes enriching PHB-producing methanotrophic strains, using a bioreactor, where ammonium includes a nitrogen source, where growth of the enriched PHB cells on ammonium selects for a PHB-producing methanotrophic strains by inhibiting survival of Type I organism growth, growing the enriched PHB-producing strains, using the bioreactor, on nitrate or urea to promote rapid and more dense growth of the enriched PHB-producing strains, where production of the PHB occurs when nitrogen is exhausted, and cycling between the enriching PHB-producing methanotrophic strains and growing the enriched PHB-producing strains, using the bioreactor, where a mixed culture of the PHB-producing strains are maintained without reducing growth rates of the methanotrophic strains or PHB production rates in the bioreactor.
priorityDate 2012-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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