http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015133336-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_99cf71d785acaa3cb42fbdfeb5ee11b2 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5306 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6845 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2015-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2bb4972a56e3a3e79d815de81e9a374c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6a4c3eda8fb1b16df3255da95bb8314d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d03e8fbf0bea8941b43a193ef673cce |
| publicationDate | 2015-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2015133336-A1 |
| titleOfInvention | Methods for determining ligand binding to a target protein using a thermal shift assay |
| abstract | The present invention concerns a method of determining whether a non-purified sample contains a target protein bound to a ligand of interest comprising the steps of a) exposing the non-purified sample to a temperature which is capable of causing or enhancing precipitation of the unbound target protein to a greater extent than it is capable of causing or enhancing precipitation of the target protein bound to the ligand; and b) analysing said sample for the presence of soluble or native target protein using two or more affinity reagents capable of binding to said soluble or native target protein with a higher affinity than to an unfolded and/or insoluble form of said target protein. The invention particularly concerns the use of two affinity reagents (e.g. antibodies) which are capable of distinguishing between soluble or native, and unfolded and/or insoluble forms of a target protein and whose detection e.g. by FRET based technology, allows the performance of the method without a separation step. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11307128-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10900879-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017118997-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11698361-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10641776-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2020158614-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017030606-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9528996-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3337905-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11567074-B2 |
| priorityDate | 2011-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 722.