| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_943d21326a8034afdb808ab726293c7d |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-64 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate |
2014-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ec5b45e80f0acb4b96a7444eb9271cd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ef53dc7e3f5a58eb23786cac8d994fd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_20dcc84536261038a1c45d9c8c40786d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_22d54c8b7e0c9bc4ada583c1b66ee26d |
| publicationDate |
2015-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2015118693-A1 |
| titleOfInvention |
Method of high-throughput sorting of high expression cell and cells sorted therefrom |
| abstract |
The invention provides a method of high-throughput sorting of high expression protein-producing cell, which utilizes linking a protein and a transmembrane domain with a self-processing cleavage site and regulating the secretion of the protein or expression of protein on the cell membrane by adding self-processing cleavage enzyme inhibitor. Then, the high expression cell line can be high-throughput sorted by a detection technique. The invention also provides a recombinant nucleotide sequence and a vector used in the method and a cell sorted by the method. |
| priorityDate |
2013-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |