| abstract |
The invention provides an ultra-rapid nucleic acid amplification method performed in a flow channel. Specifically, the invention provides a nucleic acid amplification method for performing a PCR reaction by supplying a PCR sample solution to a nucleic acid amplification device comprising a serpentine channel adapted to perform at least one PCR cycle, the nucleic acid amplification device comprising a DNA denaturation temperature zone corresponding to the curved portions at one side, an annealing temperature zone corresponding to the curved portions at the other side, and an extension temperature zone positioned between the annealing and DNA denaturation temperature zones, wherein the PCR sample solution is introduced in the form of sample plugs separated by gas into the serpentine channel using a pump, the sample solution being supplied into the channel in a state such that the solution is separated by gas into a segment corresponding to one PCR cycle or smaller segments. |