Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc2b03342511abc88f60c63f2acd96d0 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2014-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d85fc2fab120fd396f13249d7961ded0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b23d1f691ec14003557c415be07302a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_305f7cffb2984b01543356140cf6fe06 |
publicationDate |
2015-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2015017635-A1 |
titleOfInvention |
Direct Capture, Amplification and Sequencing of Target DNA Using Immobilized Primers |
abstract |
Certain embodiments provide a method for capturing a genomic fragment. The method may comprise: obtaining a substrate comprising a first population of surface-bound oligonucleotides and a second population of surface-bound oligonucleotides; hybridizing a first member of the first population of surface-bound oligonucleotides to a selection oligonucleotide comprising a region that hybridizes with the first member and a region that contains a genomic sequence; extending the first member of the first population of surface-bound oligonucleotides to produce a support-bound selection primer that comprises a sequence that is complementary to the genomic sequence; hybridizing the support-bound selection primer to a nucleic acid fragment comprising the genomic sequence; extending the support-bound selection primer to produce an extension product that contains a sequence that flanks the genomic sequence, e.g., in a genome; and amplifying the extension product on the substrate. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10968447-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10876108-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11708574-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021231263-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9957549-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10036012-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11486004-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9745614-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11098357-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9822408-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9650628-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11697843-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11099202-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10760123-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11339431-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11028430-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2612412-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10752946-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10570448-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018161019-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11725241-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9206418-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021155374-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10619206-B2 |
priorityDate |
2010-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |