http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015011008-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a59d528ec1863ff7fa28a974c1c000ed http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_716e9a2606ffd4ba1a2a3917e4a402fd |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61N1-0412 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8206 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2014-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a5e95b16e8867d4fd7fbc21eb9d2460 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ffb675b6b4a364eb79e44d1fdd0161b3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c46ab8974fb96c5bf61546cde2c8e8d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_003472de25ef7d10b122245189faf981 |
| publicationDate | 2015-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2015011008-A1 |
| titleOfInvention | Method of transferring gene into algal cell involving utilizing multiple square-wave pulses in three steps |
| abstract | To provide a technology that solves the above-mentioned problems, and is directly applicable to eukaryotic algal cells with cell-wall, the technology enabling gene transfer and transformation to be performed with high efficiency and good reproducibility irrespective of species of algae. Also provided is a method of transferring an exogenous gene into a eukaryotic algal cell, the method comprising performing electroporation using multiple square-wave pulses to the solution containing a cell of a green alga with cell-wall, and a nucleic acid molecule by the following three steps: applying a square-wave electric pulse (first electric pulse) with a high voltage for a short period of time under the condition that its total electric energy falls within a predetermined range; then applying a square-wave electric pulse (second electric pulse) with a low voltage for a long period of time two or more times; and then applying a square-wave electric pulse (third electric pulse) that is opposite in polarity to the second electric pulse, with a low voltage for a long period of time, two or more times. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2017135101-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017122189-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020049572-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-4043570-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10767201-B2 |
| priorityDate | 2013-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.