| abstract |
The invention relates to a system and method for obtaining a cellular fraction enriched with defined cells effective in promoting a defined cellular response from an in vivo cellular sample comprising: a collection tube for collecting a cell suspension ( 1 ), an anticoagulant portion ( 4 ), a portion of separation gel ( 3 ), a first collection syringe for collecting a cell depleted fluid ( 10 ), a second collection syringe for collecting the enriched cellular portion ( 11 ) and at least one needle ( 1 O) for attaching to each syringe. The system is closable to the atmosphere, further wherein the cell suspension collection tube contains the gel ( 3 ) and the anticoagulant ( 4 ), the tube is adapted such that, when containing cell suspension and centrifuged after treatment yields separation fractions, a first fraction comprising cellular fluid ( 6 ), a second fraction comprises gel layer ( 3 ), a third fraction comprises enriched cellular portion ( 7 ) on top of the gel layer and a fourth fraction comprises a cell poor portion ( 8 ). |