| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_42e94091a4fc81b8d0237117707cc7ee |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-21 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-248 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-24 |
| filingDate |
2014-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1ef240d8b4a2eb62e210c935382bd2f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_244af5ad96cd463be5ffee829e7d03cd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d65d2ab29459f0d44e3974d7fe21f519
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_238566c4b35207b29547354f76703a7a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7383e4c10b96659a16d476914866dba5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4e9c7ca971d3eebc17567c0cd1fae84c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_225e96444362ae79ac2095a89c5a5b14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56ceb98d749bade8babe242b4b01f069 |
| publicationDate |
2014-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2014273093-A1 |
| titleOfInvention |
Methods of producing antibodies in yeast |
| abstract |
The present invention describes a method for producing an antibody in Pichia pastoris, such as by fed-batch fermentation. The method may include a strategy of increasing the ethanol concentration to 18-22 g/L and then maintaining the ethanol level at 5-17 g/L to stabilize the cell mass and enhance the production rate of the antibody. The method may also include the addition of 2.0-5.0 g/L of hydroxyurea during the fermentation process to sustain a constant cell density and enhance the whole broth titer of the antibody. The method may further include a respiratory quotient control for monitoring the ethanol profile and to improve the quality of the antibody by, for example, eliminating clipping of the heavy chain. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109554314-A |
| priorityDate |
2013-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |