| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e41651508566f0385833e1ba14a21ffd |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-513
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-165
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-137
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-537
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2533-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-102
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-518 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B50-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y30-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2013-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d1aaa072eef84b6e4c143eddaee40c0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_36f49266d17aec340748990a9ff316a9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e52e05d70ad455bdb7df2457f5d7806a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a338da9c0db235ed224e03b769ac427 |
| publicationDate |
2014-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2014248610-A1 |
| titleOfInvention |
Reagents, Methods, and Libraries for Bead-Based Sequencing |
| abstract |
The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled extension probes containing phosphorothiolate linkages. In addition, the invention includes performing multiple sequencing reactions on a single template by removing initializing oligonucleotides and extended strands and performing subsequent reactions using different initializing oligonucleotides. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11035854-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11693001-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10746734-B2 |
| priorityDate |
2005-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |