http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2014154807-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bec943d88d662228f85a8cf0e248bf3c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0769a1fde962c4b67b8ec7d6520f0f92 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1058 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-385 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 |
| filingDate | 2014-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5152c3446035acf1d1d2dc4d2e3a7d31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6cc2aa9dae7a02c08b0aa3970d0d1d1 |
| publicationDate | 2014-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2014154807-A1 |
| titleOfInvention | Method for homologous recombination |
| abstract | The present invention discloses a method to construct eukaryotic cells having a target sequence in a chromosomal DNA sequence replaced by a replacement sequence of interest comprising:n modifying a parent eukaryotic cell with a preference for NHR to provide a eukaryotic cell having an increased HR/NHR ratio as compared to the parent cell, providing two sets of DNA molecules of which the first set comprises DNA molecules each comprising a first non-functional fragment of the replacement sequence of interest flanked at its 5′-side by a DNA sequence substantially homologous to a sequence of the chromosomal DNA flanking the target sequence and the second set comprises DNA molecules each comprising a second non-functional fragment of the DNA replacement sequence of interest overlapping with the first non-functional fragment and flanked at its 3′-side by a DNA sequence substantially homologous to a sequence of the chromosomal DNA flanking the target sequence, wherein the first and second non-functional fragments become functional upon recombination; transforming the modified eukaryotic cells with both sets of DNA molecules; and growing the cells to obtain transformed progeny cells having a DNA molecule from the first set recombined with a DNA molecule from the second set into a DNA molecule comprising a functional replacement sequence and having the target sequence replaced by the functional replacement sequence. |
| priorityDate | 2007-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 635.