| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_19dd0d889cedfe1b171584512ae9edaa |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B03D1-1462
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B03D2203-003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B03D1-028 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B03D1-1468
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B03D1-082
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B03D1-1487
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B03D1-18 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2014-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ef860bd9c0e47ccc9b7997756531ad5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5231641e0b7aa9a70c738a2913c487b9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06a488ace3b149411b1cbd35ca355eda |
| publicationDate |
2014-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2014154747-A1 |
| titleOfInvention |
Methods and devices for producing biomolecules |
| abstract |
A scalable process and device for producing a bio molecule, in particular pharmaceutical grade plasmid DNA is described. The process includes the steps of alkaline lysis, neutralization and clarification and can be further extended. For separating the lysate and the precipitate an improved floatation method is disclosed. This method is based on attachment of CO 2 bubbles on the precipitate floe. The CO 2 is released from a carbonate salt during or after neutralization (acidification). The method of the invention is preferably carried out in an automated continuous mode applying devices for lysis and neutralization and a novel device for completely continuous clarification (separation of flocs and clarified lysate). |
| priorityDate |
2008-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |