Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cf32b60391b469c41e86c353437f5751 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_537adc85bf69e41fb4976932b14d739e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_527cdfae12daa9fe53bc56cf17089a04 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-38 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 |
filingDate |
2014-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_937a6aacbf3a32a75016200334f1ea9b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10a71c30696ca1492f55eecfaace5b16 |
publicationDate |
2014-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2014154739-A1 |
titleOfInvention |
Methods for selecting eukaryotic cells expressing a heterologous protein |
abstract |
The invention pertains to a method for selecting at least one eukaryotic host cell expressing a product of interest, comprisingn (a) providing a plurality of eukaryotic host cells, wherein cellular viability of said host cells is dependent upon folate uptake, wherein said host cells comprise at leastn (i) a foreign polynucleotide encoding a product of interest and (ii) a foreign polynucleotide encoding a DHFR enzyme; n (b) culturing said plurality of eukaryotic host cells in a selective culture medium comprising at least an inhibitor of DHFR and folate in a limiting concentration; and (c) selecting at least one eukaryotic host cell expressing the product of interest. nn Also provided is a method for expressing a product of interest which is based on host cells selected by said method and a cell culture medium. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11174494-B2 |
priorityDate |
2009-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |