Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ad2cd796f8c4dcb425ddcc68707d3444 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_843e3d073c9dbcae1f7c273466022a0f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_faf522b8b83eded745c73018576219ba |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
filingDate |
2014-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f5e10256a97024b0bff3d3c5b32c496 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2ba102718a14849f4831b49d802968a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_901daf6c78a57a54e9b4f72389ddb3c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3b6dad531c910cf5b9b3f77741f9894 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_845199677f4e99f0081c5ec31d0e2e61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d49e169e006f5e0f1c7d7e5d3941624d |
publicationDate |
2014-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2014141416-A1 |
titleOfInvention |
Method for highly sensitive detection of protein-protein interaction |
abstract |
The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex. |
priorityDate |
2009-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |