http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2014134679-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ac3990a96e5fce2df037fb291eef2e59 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_31a4849bb664dd1faf516187b1d0c741 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ab37513bc4d1c3e6bdd4754e5e75a7ef |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04 |
filingDate | 2012-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d273b44d7d9147c2fff3e737d17afcff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e025c86b54b629b2b353f1aa8164ac56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be4abc264dc90095d5b180bf90a1d2d5 |
publicationDate | 2014-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2014134679-A1 |
titleOfInvention | Method for producing polysaccharide |
abstract | The present invention aims to provide a method for producing a polysaccharide with high efficiency using a polysaccharide synthase. The present invention provides a method for producing a polysaccharide, including allowing polysaccharide synthase (B) to act on ribonucleoside diphosphate-monosaccharide (A) shown below to produce a polysaccharide, wherein in 10 to 100% of the duration in which (B) acts on (A), the concentration of ribonucleoside diphosphate in a reaction solution is lower than 100 times an inhibitory concentration IC 50 described below against polysaccharide synthase (B). n Inhibitory concentration IC 50 : a concentration of ribonucleoside diphosphate at which an enzyme activity of polysaccharide synthase (B) is reduced by half while under a condition where (B) has a concentration at which (B) acts on ribonucleoside diphosphate-monosaccharide (A), wherein ribonucleoside diphosphate-monosaccharide (A) is used as a substrate and ribonucleoside diphosphate is used as an inhibitor. n Ribonucleoside diphosphate-monosaccharide (A): a sugar nucleotide in which a proton of at least one hydroxyl group of at least one monosaccharide (a) selected from the group consisting of triose (a-1), tetrose (a-2), pentose (a-3), hexose (a-4), heptose (a-5), and monosaccharide (a-6) described below is substituted with a functional group represented by any one of chemical formulae (1) to (5) below. n Monosaccharide (a-6): a monosaccharide selected from the group consisting of (a-1), (a-2), (a-3), (a-4), and (a-5), in which at least one member selected from the group consisting of a proton, a hydroxyl group, and a hydroxymethyl group of the monosaccharide is substituted with substituent (E) described below. n Substituent (E) is at least one substituent selected from the group consisting of carboxyl, amino, N-acetylamino, sulfate, methylester, N-glycolyl, methyl, 1,2,3-trihydroxypropyl, phosphate, and 2-carboxy-2-hydroxyethyl groups. |
priorityDate | 2011-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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