| abstract |
Disclosed herein is a method for detecting an antigen. The method includes: reacting an antigen as an analyte having an absorption wavelength at 300 nm to 400 nm with an antibody to form an antigen-antibody conjugate; irradiating light to the antigen-antibody conjugate to induce fluorescence resonance energy transfer, thereby obtaining a fluorescence spectrum; and determining the presence of the antigen as the analyte by analyzing the fluorescence spectrum, and then measuring the concentration of the antigen. The method is capable of directly detecting various antigens in a homogeneous liquid state, which induces no competitive reaction, with high sensitivity and high selectivity through fluorescent resonance energy transfer, without any modification. |