| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_75b765b53e6651e9863a3a35e4c1e164 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S977-906
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S977-811 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-48721
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M33-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N13-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-14 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N13-00 |
| filingDate |
2013-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77d1ee0c005c34e9ac005fa8a0ed8ac7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01889e9bf0c0453a93c6d6bec418edb0 |
| publicationDate |
2014-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2014113356-A1 |
| titleOfInvention |
Nano-electrode based chip |
| abstract |
The present invention disclosed a nano-electrode based transparent chip, which is applicable to perform a micro fluidic substance diffused through a specific region of a single cell membrane by localized electroporation technique with membrane reversibility. The chip comprises a silicon-based layer, different structural layers, an insulating layer and a micro fluidic layer. When the individual single cell was placed on the gap between a plurality of triangular-shaped nano-electrodes with nano-tip, then electric field can intense on a specific region of the individual single cell causes the formation of nano-pores on the membrane, resulting to deliver drugs, DNA molecules from outside of the cell to the inside of the cell with a high transfection rate and a high cell viability. This technique not only generates well-controlled nano-pores to allow rapid recovery of the cell membrane, but also provides clear optical path for potentially monitoring/tracing the drugs into the cell. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11225638-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017176764-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11371004-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018200873-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11198841-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2016341688-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110741076-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9683956-B2 |
| priorityDate |
2012-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |