http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2014065699-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_41583b9989764ebec3d9b73aa2ea1278 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d7607e1de4c54a2b600d667457b5e268 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_18d92c46f60af8af24e262b67381d7eb |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-71 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-71 |
| filingDate | 2012-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_007b66937fce320afd548591cd45b31d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70d9d05cf26cb5e27d4a347f59275441 |
| publicationDate | 2014-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2014065699-A1 |
| titleOfInvention | Novel bacterial expression plasmid |
| abstract | The present invention provides expression vectors useful for high-throughput screening of gene libraries. In a specific embodiment, an expression vector comprising (a) the Rop gene operatively linked to the trp promoter-operator; (b) a purification tag sequence and a protease cleavage site downstream of the Rop gene; and (d) a multiple cloning site downstream of the protease cleavage site, wherein the insertion of a heterologous gene of interest into the multiple cloning site and subsequent expression thereof in a host cell produces a high yield of a fusion protein comprising the Rop protein and the protein encoded by the heterologous gene of interest without the need of chemical inducers, temperature shifts, or growth medium alterations to initiate protein synthesis, and wherein the fusion protein controls plasmid replication at temperatures below about 30° C. but exhibits runaway plasmid replication when cultured at about 37° C. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111621854-A |
| priorityDate | 2011-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 163.