Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f64ddff6bf2f09c22c2ef1a20f26a91c |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6832 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-483 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
filingDate |
2013-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92abf5731f82cab89aab39c348f8f402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f9e9831ccd4d7083054cd423a04940c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_886133f571d4f5fa340db8ebcda0f506 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b71d532a2d92b739eb5b0171114485d1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de66c41e6abd0dd8a283ee006070e011 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9dd4029e80d61741bc6e140156052116 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e21ef57c4a11e31a7689fe93b5e85f8e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a7b3ed7b6d22b247f95a7580eb4cfa4 |
publicationDate |
2014-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2014057269-A1 |
titleOfInvention |
Materials and Methods for Detection of Nucleic Acids |
abstract |
Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10752939-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10975419-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016025452-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9982291-B2 |
priorityDate |
2000-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |