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publicationDate 2014-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2014011254-A1
titleOfInvention Nuclease Reduction
abstract A method for reducing nuclease activity in a subtilisin solution obtained from a fermentation broth comprisingn a) adjusting the pH of the subtilisin solution to a pH in the range of from pH 8.5 to pH 10.5; b) adding a polyol; c) adjusting the temperature of the subtilisin solution to a temperature in the range of from 50° C. to 80° C.;n nwhereby the nuclease activity is reduced to less than 5% of the initial value and the subtilisin activity is maintained at more than 80% of the initial value.
priorityDate 2007-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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