http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2013345075-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_35c349407fd1866558fcaa697fd5b353 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d394e3a458d9c31ac77246f6aec69865 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9abe3c5db943bda55e09f7baecde3962 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y30-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 |
filingDate | 2013-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91ee0df18f9d0c4c6d622bf26bc4550b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de8368b48b77f6ea904d39b21f85bfc3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab241e784805147d734a19a551d8e737 |
publicationDate | 2013-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2013345075-A1 |
titleOfInvention | Tissue Preservation and Fixation Method |
abstract | This invention relates, e.g., to a composition that, at room temperature, when contacted with a sample comprising phosphoproteins, can fix and stabilize cellular phosphoproteins, preserve cellular morphology, and allow the sample to be frozen to generate a cryostat frozen section suitable for molecular analysis. The composition comprises (1) a fixative that stabilizes the proteins in the sample and that has a sufficient water content for a stabilizer and/or a permeability enhancing agent to be soluble therein; (2) a stabilizer, comprising (a) a kinase inhibitor and (b) a phosphatase inhibitor and, optionally, (c) a protease (e.g., proteinase) inhibitor; (3) a permeability enhancing agent; and (4) lactic acid. Methods and kits are described for preserving phosphoproteins, using such a composition. Also described are endogenous surrogate markers for monitoring protein degradation, including the loss of posttranslational modifications (such as phosphorylation), e.g. following removal of a cell or tissue from a subject; and exogenous molecular sentinels (e.g. phosphoproteins attached to magnetic nanoparticles) that allow one to evaluate the processing history of a cellular or tissue population sample. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2017229088-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017155869-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11226271-B2 |
priorityDate | 2006-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1481.